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KMID : 0903520090520030290
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 3 p.290 ~ p.294
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Isolation of a Calcium-binding Peptide from Enzymatic Hydrolysates of Porcine Blood Plasma Protein
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Lee Seung-Hwan
Song Kyung-Bin
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Abstract
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A calcium-binding peptide was purified from hydrolysates of porcine plasma protein. Porcine plasma protein was hydrolyzed using Flavourzyme, and the hydrolysates were filtered using YM-3 membrane. The membrane filtered solution was fractionated using Sephadex G-15, normal phase high-performance liquid, ion exchange chromatography, and fast protein liquid chromatography for a calcium-binding peptide. The sequence of the purified calcium-binding peptide was analyzed using liquid chromatography/electron spray ionization tandem mass spectrum and identified to be Val-Ser-Gly-Val-Glu-Asp-Val-Asn. The calcium- binding peptide can be used as a food ingredient or feed for calcium deficiency.
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KEYWORD
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calcium-binding peptide, enzymatic hydrolysates, porcine blood plasma protein, purification
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